. 2015 Apr 13;10:2895–2911. doi: 10.2147/IJN.S76312

Table 2.

The stimulation index of T lymphocyte proliferation in specific pathogen-free chickens post-immunization

Group	Stimulation index
Group	7 days post-immunization	14 days post-immunization	21 days post-immunization	28 days post-immunization	35 days post-immunization	42 days post-immunization
pFDNA-LDH@SiO₂-NPs IN	0.522±0.021^a	0.689±0.029^a	1.110±0.019^a	1.444±0.039^a	1.866±0.07^a	2.056±0.042^a
pFDNA-LDH@SiO₂-NPs IM	0.517±0.003^a	0.690±0.014^a	1.104±0.028^a	1.234±0.071^b	1.745±0.096^b	1.803±0.028^b
Naked plasmid pVAX1-F(o) DNA IM	0.515±0.031^a	0.671±0.017^a	1.009±0.007^a	1.228±0.057^b	1.566±0.037^c	1.606±0.025^c
Blank LDH@SiO₂-NPs IM	0.402±0.007^b	0.478±0.004^b	0.727±0.075^c	0.805±0.047^c	1.121±0.281^d	1.044±0.055^d
PBS IM	0.364±0.03^b	0.441±0.030^b	0.701±0.056^c	0.743±0.028^d	1.100±0.021^d	1.008±0.023^d

Notes: Values are presented as mean ± standard deviation of five experiments in each group. Values within the same column with the different superscript letters are significantly different (P<0.05; Student’s t-test).

Abbreviations: IM, intramuscularly; IN, intranasally; LDH, layered double hydroxide; NPs, nanoparticles; PBS, phosphate-buffered saline; pFDNA-LDH@SiO₂-NPs, Newcastle disease virus F gene encapsulated in LDH@SiO₂-NPs.