Table 1.

GEP DNA copy number with reference to A.) centromere 17 (CEN17) ¹ and B.) centromere 3 (CEN3) ² by FISH analysis ^3,4

A.	GEP	CEN17	GEP	CEN17
HCC	RP11-436 J4	pEZ17-4	RP11-52 N13	pEZ17-4
801	3.66	3.37	3.80	3.51
884	3.08	2.92	3.02	2.77
B.	GEP	CEN3	GEP	CEN3
HCC	RP11-436 J4	pAE0.68	RP11-52 N13	pAE0.68
801	3.73	2.17	3.73	1.97
884	2.85	2.10	2.89	1.92

¹GEP DNA copy number with reference to centromere 17 (CEN17). In these two cases, CEN17 scores ranged 2.92 to 3.51 per cell, and similarly GEP scores ranged 3.02 to 3.80 per cell. The data indicated an increased chromosome 17 copy number, at centromere and GEP locus at 17q21, in these cases.

²GEP DNA copy number with reference to centromere 3 (CEN3). CEN3 scores ranged 1.92-2.17 per cell, indicating approximately two copies of chromosome 3. The number of GEP DNA per 2 centromere (diploid) was 3.60 and 2.86 for HCC801 and HCC884, respectively, by FISH analysis. GEP copy number was 2.56 and 2.93 for HCC801 and HCC884, respectively, by QuMA (reference to D3S1609).

³DNA copy number for each set was quantified for 100 cells and data presented per cell.

⁴Noted independent platform using QuMA qPCR, both HCC801 and HCC884 showed similar increased GEP DNA copy number.