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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Curr Protoc Hum Genet. 2015 Apr 1;85:13.11.1–13.11.9. doi: 10.1002/0471142905.hg1311s85

Table 1.

Formula for viral infection

Name Vp/ml or
pfu/ml		 Cell/wells MOI Wells Amount (mL) Virus titer 100 dil. titer Add amount(μL) in
E_ ml
virus unit The number
of cells in one
well		 Multiple of infection

: how many viruses per
cells		 Number of total
wells to use		 Amount of the
total media
needed		 Virus titer to
be used		 100 fold diluted
virus titer		 Required Amount of
100 fold diluted
virus
A B C D E F
= B × C × D 		G
= A/100		 H
=F / G × 1000
Ex. You want to use 1.012 virus to infect 3 well of 2X104 cells with 1000 MOI
JK 1.0×1012 2.0×104 1000 4wells
(3 wells + 1 extra)		 2mL
(500μL X4well)		 8.0×107 1.0×1010 8μL
→ You can add 8μL of 100 fold diluted stock into 2ml of infection media. Then add 500μL to each well to achieve 1000 MOI virus infection.