Figure 7. Haematopoietically derived cells acquire β-gal from LECs and induce Bg2 anergy.

(a) CTV-labelled Thy1.1⁺ Bg2 cells were transferred into the indicated recipients, along with CTV-labelled Thy1.1^neg cells as an injection control. Proliferation in skin-draining LNs was measured 7 days later. Data representative of 1–2 experiments with 2–5 mice each. (b) CTV-labelled Thy1.1⁺ Bg2 cells were transferred into the indicated recipients, and T_reg development was measured 3 days later. Data representative of 2 experiments with 2–3 mice each. (c) Experimental design to measure tolerance induction. CTV-labelled CD25^negThy1.1⁺ Bg2 CD4 T cells were adoptively transferred, and recipient mice were challenged 28 days later with peptide-pulsed BMDCs. Proliferation was measured 5 days later by Ki67 upregulation, and FoxP3 and CD25 were measured to assess T_reg formation. (d) Representative and (e) cumulative data gated on CD4⁺Thy1.1⁺ Bg2 cells from skin-draining LN from 2 experiments with 1–7 mice each. *P<0.05; **P<0.01; ***P<0.001, NS=not significant. Indicated comparisons were made using a one-way analysis of variance with Bonferroni post-test. All data shown as mean±s.e.m.