Fig 3. Ube3a is not imprinted in cultured astrocytes.

A) Representative data from enriched astrocyte cultures showing expression of Ube3a and high expression of GFAP, confirming astrocyte enrichment. B) Quantification of data showing an approximately 50% reduction in Ube3a in cultured astrocytes from AS animals. This is not different from 50% as determined by a one sample t-test against 50%, the expected reduction for a biallelically expressed protein. n = 6–7 independent cultures from individual animals per group.