Table 1. Characterization of the sites of Tn5-B22 transposition in Y. enterocolitica mutants.
Mutant | site of transposon insertion a | protein (function) | regulation d |
---|---|---|---|
AR22 | YE105_C2078 b | TyrR (DNA-binding transcriptional | + |
regulator) | |||
AR24 | YE105_C1595 b | FimA (pilus assembly protein) | + |
AR33 | YE105_C0917 b | general secretion pathway protein J | + |
(type II secretion system) | |||
AR53 | YE105_C3385 c | glycosyl hydrolase family protein | + |
AR83 | YE105_C1163 b | DsrE (oxidative sulfur metabolism) | - |
AR202 | intergenic region | hypothetical proteins (unknown | + |
(YE105_C2160, | function) | ||
YE105_C2160) c | |||
AR704 | YE105_C2117 b | hypothetical protein (homolog of | - |
tellurite resistance protein TerB) |
a sequence of region flanking transposon insertion comes from the genome of Y. enterocolitica subsp. palearctica 105.5R(r) (NC_015224) deposited in the NCBI database.
b lacZ is in the same orientation as the region with similarity to the database entry.
c lacZ is in the opposite orientation to the region with similarity to the database entry.
d + or-mean positive or negative OmpR-dependent regulation.