Table 1.

Comparison of oxygen binding parameters and cation composition of haemolymph between Pareledone charcoti , Octopus pallidus and Eledone moschata

Temperature (°C)	P 50 (kPa)	Δmmol l −1 O 2 /ΔpH	Bohr coefficient* (pH range 6.7-7.5)	Δ P 50 (kPa)/°C #	Cation concentration (mmol L −1 )
			(Δlog P 50 /ΔpH)		Mg 2+	Na +	K +	NH 4+	Ca 2+
Pareledone charcoti
0	0.41	1.17
	(NA, pH 7.42)	(0.88-1.46)
5	0.7	2.39	−1.22	0.15	52.6	385.5	8.5	6.1	8.8
	(NA, pH 7.34)	(0.47-4.32)	(−1.67 - -0.78)	(NA)	(39.8 – 65.5)	(348.4 – 422.6)	(6.7 – 10.2)	(1.6 – 10.5)	(7.3 – 10.2)
10	2.34	3.17
	(2.19-2.49, pH 7.27)	(2.51-3.82)
Octopus pallidus
10	1.63	1.40
	(0.99-2.26, pH 7.27)	(1.26-1.55)
15	1.81	2.55	−1.97	0.39	45.6	372.0	10.0	8.6	7.5
	(1.46-2.16, pH 7.19)	(2.24-2.85)	(−2.50 - -1.44)	(0.05 – 0.72)	(41.6 – 49.6)	(356.2 – 387.8)	(7.7 – 12.3)	(3.2 – 13.9)	(6.7 – 8.2)
20	6.07	2.94
	(3.74-8.40, pH 7.11)	(2.63-3.25)
Eledone moschata
10	0.56	1.13
	(0.1-1.02 pH 7.27)	(0.77-1.48)
15	1.49	1.79	−1.88	0.21	49.9	386.8	8.8	3.0	7.3
	(NA, pH 7.19)	(0.70-2.89)	(−2.22 - -1.55)	(0.11 – 0.30)	(42.2 – 57.6)	(334.0 – 439.5)	(6.2 – 11.4)	(−3.8 – 9.8)	(5.7 – 8.9)
20	2.62	2.08
	(2.00-3.24, pH 7.11)	(1.79-2.37)
ANOVA†		F 2, 149 = 10.82	F 2, 16 = 3.71	F 2, 7 = 1.58	F 2, 17 = 0.78	F 2, 17 = 0.31	F 2, 17 = 0.85	F 2, 17 = 1.35	F 2, 17 = 2.28
		P  < 0.001	P  = 0.048	P = 0.271	P = 0.475	P = 0.739	P = 0.446	P = 0.285	P = 0.133

Numbers in brackets indicate the range of 95% confidence intervals.

*Data at different temperature were pooled for each species.

^#Based on alpha-stat shifted venous pH and a 10°C temperature interval.

†ANOVA results for between species comparison.