Table 2.
Variability of the intron-1 of the kdr gene in Anopheles coluzzii and Anopheles gambiae collected in 2011 in Douala and Yaoundé
| Mutations positions | Unpolluted | Polluted | Cultivated | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 702 | 703 | 896 | Others | Haplotypes | An. coluzzii | An. coluzzii | % | An. coluzzii | An. gambiae | |||
| 2 N = 30 | % | 2 N = 18 | % | 2 N = 22 | % | 2 N = 16 | % | |||||
| T | C | C | - | MS1 | 16 | 53.3% | 8 | 44.4% | 4 | 18.2% | 16 | 100% |
| T | C | A | - | M14 | - | - | 2 | 11.1% | 2 | 9.09% | - | - |
| C | C | C | - | MS3 | 6 | 20% | 6 | 33.3% | 12 | 54.5% | - | - |
| C | C | C | 169C | M15 | 4 | 13.3% | - | - | - | - | - | - |
| C | C | C | 394G | M16 | 4 | 13.3% | 2 | 11.1% | 4 | 18.2% | - | - |
| Kdr alleles- Intron-1 haplotypes | ||||||||||||
| MS1-L1014L | 6 | 40% | 3 | 33.3% | - | - | 1 | 12.5% | ||||
| MS1-L1014F | 2 | 13.3% | 1 | 11.1% | 2 | 18.2% | 6 | 75% | ||||
| MS1-L1014S | - | - | - | - | - | - | 1 | 12.5% | ||||
| MS3-L1014L | 3 | 20% | 3 | 33.3% | 6 | 54.5% | - | - | ||||
| M14-L1014L | - | - | 1 | 11.1% | 1 | 9.1% | - | - | ||||
| M15-L1014L | 1 | 6.67% | - | - | - | - | - | - | ||||
| M15-L1014F | 1 | 6.67% | - | - | - | - | - | - | ||||
| M16-L1014L | 2 | 13.3% | 1 | 11.1% | 2 | 18.2% | - | - | ||||
| Estimates of genetic variability | ||||||||||||
| h | 4 | 4 | 4 | 3 | ||||||||
| Hd ± SD | 0.82 ± 0.034 | 0.76 ± 0.05 | 0.59 ± 0.085 | 0.43 ± 0.14 | ||||||||
| k | 1.42 | 1.098 | 1.048 | 0.633 | ||||||||
| n | 4 | 3 | 3 | 2 | ||||||||
| D | 1.045 | 0.718 | 0.706 | 0.13 | ||||||||
| Fs | −0.614 | 0.089 | 1.43 | 0.062 | ||||||||
h: number of different haplotypes; Hd ± SD, haplotype diversity (±standard deviation); K: average number of pairwise nucleotide differences; n: number of mutations; D: Tajima’s test; Fs: Fu’s Fs statistic.