Table 2. Summary of metabolic genome partitions into essential and nonessential genes according to Essential Gene Sets.

Partition Name	‘essential’ set	‘nonessential’ set
AE-partition	AE genes	All-but-AE genes
APE-partition	APE genes	NE genes
AENE-partition	AE genes	NE genes

Three methods are presented for partitioning the genome into essential and nonessential gene according to Essential Gene Sets:

AE-partition is equivalent to the partition done in previous related experimental research, had the experimental essential gene set been determined on a rich medium.

APE-partition is similar to the partition done in previous related experimental research, had the experimental essential gene set been determined on a poor medium.

AENE-partition produces a marked separation, possibly helping overcome metabolic model inaccuracy. AE is the core group of essential genes with a higher probability of aligning with essential genes from experimental data. Similarly, NE are more likely to overlap with nonessential experimental genes. This partition does not cover all metabolic genes, leaving out the PE set.