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. 2015 Apr 20;10(4):e0124708. doi: 10.1371/journal.pone.0124708

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© 2015 Liao-Chan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Fluorescence of Alexa Fluor 488 (A488) on microbeads coated with 1C1-A488 or PC-3 cells stained with 1C1-A488 was quenched with a titration of the benchmark, a rabbit anti-A488 polyclonal, or 1 of 3 anti-A488 mAbs. One representative experiment of multiple is shown. (B) Fluorescence of Alexa Fluor 594 (A594) on microbeads coated with 1C1-A594 or PC-3 cells stained with 1C1-A594 was quenched with a titration of 1 of 3 anti-A594 mAbs. One representative experiment of multiple is shown. (A, B) Median fluorescence intensities (MFIs) at each anti-A488 or anti-A594 mAb concentration were normalized against a buffer control. The chimeric IgG1 isotype control was used as a non-quenching mAb control. The IC50 values (microgram/ml) of quenching and the corresponding 95% confidence intervals (95% CI) are listed for both the microbead- and cell-based titrations.