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Impairing actin dynamics decreases thermotolerance in mammalian cell culture. (A) HEK293T cells treated with Cytochalasin D or Latrunculin A show disrupted filamentous actin formation in mammalian cell culture. (B) Both Cytochalasin D and Latrunculin A treatment reduce the thermotolerance of HEK293T cells after a heat shock of 45°C for 2 hours. (C) Proposed model of the duel pathways of HSF-1 mediated health assurance. *P < 0.05; error bars indicate SEM; scale bars indicate 5 μm.
