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. 2015 Apr 9;8:211. doi: 10.1186/s13071-015-0816-3

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© Yuan et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Knockdown of the TMEM63A gene and rHco-gal-m-treatment affected the PBMC proliferation. PBMC can be share as blank group (group 1), ns siRNA group (group 2), ns siRNA/g group (group 3), 63A siRNA group (group 4) and 63A-siRNA/g group (group 5), which were incubated with equal volume reduced serum medium (group 1), ns siRNA (group 2 and 3) and TMEM63A-siRNA-1 (group 4 and 5) for 60 h. RHco-gal-m in all RNA interference groups (group 3 and 5) was added 12 h before the end of RNA interference period. The proliferation was measured by CCK-8 incorporation. Cells proliferation index was calculated considering the OD450 values in blank group as 100%. Results presented here were collected from one independent experiment and were representative of three independent experiments. Data were represented as mean ± SD, n = 6; ^*p < 0.001 versus the ns siRNA group; an asterisk and a capped line designates two groups differ significantly (p < 0.001).