Table 6.
The 23 Aedes albopictus microsatellite loci validated in this study
| Locus | Type 1 | Repeat motif | Primer sequences (5’ to 3’) 2 | T a (°C) 3 |
|---|---|---|---|---|
| Aealbmic1 | P | (TC)10 | F: [6FAM]ACCTTGCGTAGGATGACGAT | 60 |
| R: GCCATGATCACGAGCCTATT | ||||
| Aealbmic2 | I | (GTT)5TGGAGTGAG(GTT)6 | F: [6FAM]ACGATGCGTAACCATTCGAT | 58 |
| R: AACACCGCCGAATATGAAAC | ||||
| Aealbmic3 | P | (AAC)9 | F: [HEX]ACCATACAGCCTGGAGTTCG | 58 |
| R: GGGGTTGTGTGAATTGTCGT | ||||
| Aealbmic4 | P | (CAA)8 | F: [6FAM]ATCGCGGGTTTTCTATTCCT | 58 |
| R: ATCAACGAAACCGAAAGCAT | ||||
| Aealbmic5 | P | (TGT)13 | F: [HEX]AACCCATCGAACACAGAAGG | 58 |
| R: GTACGGTTGACTCGCTGTGA | ||||
| Aealbmic6 | I | (GTT)3GCT(GTT)3 GGT(GTT)4 | F: [HEX]GATGGTCCGTATTTGGGTTG | 57 |
| R: ATCTTCACTCATCCGCCATC | ||||
| Aealbmic7 | I | (TTG)8ATG(TTG)4 | F: [HEX]ATAGACGGGAGTCGGTTCCT | 58 |
| R: TCCAACCGCTAGTGTCATCA | ||||
| Aealbmic8 | P | (GT)8 | F: [HEX]TTGTTGTTCGGTTGTTGTTTG | 57 |
| R: CGGGTTCCAACTATGTACGA | ||||
| Aealbmic9 | P | (GAT)7 | F: [HEX]GCGATGACAGTGGAACAAGA | 58 |
| R: GCTTGGCAGGGAACAAATTA | ||||
| Aealbmic10 | P | (ATC)9 | F: [6FAM]ATCGCCTTCACTCTTCTTCG | 60 |
| R: CCAATCCTGAGCCGTACATT | ||||
| Aealbmic11 | P | (TGT)5 | F: [6FAM]CTCTGCGTTCCGGTTCTATC | 58 |
| R: AGGCAACCTCTCGAATGAAA | ||||
| Aealbmic12 | P | (GAT)7 | F: [6FAM]AGAGCCCTCGAAAAGAGAGC | 58 |
| R: AGCACTCATTCTTGGCTTGG | ||||
| Aealbmic13 | I | (GAT)GAC(GAT)4 | F: [6FAM]TCACACCATGGTCAAAGCAT | 58 |
| R: TGCTGAGTTGAATGGAAACG | ||||
| Aealbmic14 | I | (CT)11 T(CT)2 | F: [6FAM]CAGAAGGTCTTGGATTCACTC | 58 |
| R: TTTCCAATTCGTTTCTGGTTC | ||||
| Aealbmic15 | P | (GTT)7 | F: [6FAM]GGAATGGTTCCCTGGCTAAT | 58 |
| R: CCAACTCCGAAGAAGCCATA | ||||
| Aealbmic16 | P | (CAT)7 | F: [HEX]CACAACAACGAGAGTGTCGAA | 60 |
| R: CCGAGGGCAACACGATATAC | ||||
| Aealbmic17 | P | (CAA)15 | F: TCACCACAACAAACGGAATC | 58 |
| R: AGAGTTTTGGGCGCAGTTTA | ||||
| Aealbmic18 4 | I | (TG)12TA(TG)7 | F: GGGCCGAATCTCATATACCA | 59 |
| R: CTCAAATCGGTTGACAGACG | ||||
| Aealbmic19 | P | (ATC)9 | F: ACATATAGGTAATCCTTGCGCTGAT | 59 |
| R: GTTCCCGTATTTGATATTTGCTTT | ||||
| Aealbmic20 | P | (CA)44 | F: GGAGGCCATCATAATTTCGAC | 58 |
| R: AGTGGACCACAGACAGACCATT | ||||
| Aealbmic21 | P | (ATG)7 | F: CCCTACAGCCCTGATTGAGA | 58 |
| R: CGAGTTGGGATGTGTGATTG | ||||
| Aealbmic22 5 | I | (AC)45AT(AC)2AT(AC)5 | F: AAATCGGTTGAGGTTTACTGC | 59 |
| R: ATTCTTAGCCGGCAACTACG | ||||
| Aealbmic23 | P | (ACA)8 | F: AACGGAGCGGAGTCGATTAT | 58 |
| R: CTACTACCCGCTGCCTTCTG |
1Motif type (P: perfect repeats; I: imperfect repeats).
2The fluorescent label (6FAM or HEX) on the forward primer is indicated for the 16 loci that were subsequently used for the population analysis.
3Annealing temperature (T a).
4,5SSR sequences that correspond to previously identified loci: (alb212) [57] and (AealbD2) [58], respectively.