A) Western immunoblot analysis of SOD1 and SOD2. E2 doses in combination with 3.3 mM ascorbate: 1 μM and 10 μM. GAPDH was used as control. Numbers represent densitometric analysis compared to untreated, except 3A (top) where compared to ascorbate. B) GSH content of cells treated with ascorbate, E2 alone (10 μM), or the combination for 4 hours. Bars represent mean±SEM (n=2-3, *p<0.05, **p<0.005). C) Viability of SUM149 cells treated with the combination of E2+asc (10 μM) or BuOE2+asc (30 μM) in the presence or absence of 1500 U/mL catalase, after 24 hours. Bars represent mean±SEM (n=2-3, *p<0.05, **p<0.005).