Table 1. Characterization of Bursaphelenchus xylophilus CTLs, and primers used for CTLs expression analysis.
| Gene and Protein DB | ORF 1 | MW (Da) | SignalP 2 | Secretome 3 (Sec/whole) |
|---|---|---|---|---|
| Bxy-ctl-1 (BUX.s00579.159) | 513 | 58836.42 | NO | NO (ND) |
| Bxy-ctl-2 (BUX.s01109.377) | 272 | 54869.71 | NO | YES (0.26) |
| Primers for qRT-PCR (5’– 3’) | ||||
| Bxy-ctl-1 | For: GCCAGCGTCTTCAGCAAAGT; Rev: CCAAATTCCGTCATCGGTGT | |||
| Bxy-ctl-2 | For: CCGACTTCTTTCAACGGAAC; Rev: CCTTCATCGAGCACCTTTTC | |||
| Bxy-act-1 | For: CATCCTCCGTCTCGACTTGG; Rev: ATGTCACGCACGATTTCACG | |||
| Primers for In-situ hybridization (5’– 3’) | ||||
| Bxy-CTL-1 | For: CTTCCGATCAACTGCCCCTT; Rev: ATTTAGACAAGGGGCCAGCC | |||
| Bxy-CTL-2 | For: AGAGACCCCATCCTCTTCCC; Rev: AGCTTCTCCCTTTGCGTTGA | |||