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. 2015 Apr 15;16(1):298. doi: 10.1186/s12864-015-1494-4

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© Zhang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characteristics of the homology search of unigenes against the NR database. (a) Effects of query sequence length on percentage of significant matches. The cut-off value was set at 1.0e-5. The proportion of sequences with matches in the NR database at NCBI is greater among the longer assembled sequences. (b) Similarity distribution of the best BLAST hits for each unigene. (c) E-value distribution of the top BLAST hits for each unigene. (d) Species distribution is shown as the percentage of the total homologous sequences.