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. 2015 Apr 19;46(1):45. doi: 10.1186/s13567-015-0171-6

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© Seele et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The proteolytic rIde _Ssuis constructs modulate IgM binding to the surface of erythrocytes after incubation in αEry sera. (A, B) Flow cytometry analysis of IgM and IgG binding to ovine erythrocytes after incubation with porcine sera drawn pre, post prime or post booster vaccination with ovine erythrocytes (generation of αEry sera). (C, D) Binding of specific IgM antigen to the surface of ovine erythrocytes is significantly reduced after pretreatment of a post prime αEry serum with rIde_Ssuis and rIde_Ssuis_homologue. The post prime αEry serum was preincubated with the indicated Ide_Ssuis constructs and as a control with MRP. (A, C) The percentage of erythrocytes labelled with IgM or IgG and (B, D) the mean fluorescence intensities are shown. αEry sera were inactivated in these assays to allow flow cytometric analysis of erythrocytes. Bars and error bars represent mean values and standard deviations, respectively. Significant differences are indicated (***p < 0.001) except for differences between pre and post vaccination sera.