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. 2015 Apr 20;11(4):e1004549. doi: 10.1371/journal.ppat.1004549

Fig 5. Inhibiting miR-328 enhances bacterial clearance in cigarette smoke-exposed mice.

Fig 5

BALB/c mice were exposed to cigarette smoke daily for 8 weeks. At the completion of the model, mice were challenged with NTHi and treated with ant-328 or scrambled antagomir control 6 h post infection. (A) MiR-328 expression in lungs was determined using Taqman PCR, normalised to sno-202 and expressed as fold change compared to vehicle control (B) Bacterial load in the lungs was measured 18 h later by plating and colony counting of lung homogenates, (C) BAL fluid was collected, cells isolated by cytospin and stained, and the total cellular infiltrate was assessed. (D) Compliance and (E) elastance were measured using the flexivent system. (F) Expression of muc-5ac in lung tissue was assessed using quantitative PCR, normalised to HPRT housekeeping control and expressed as fold change over the NTHi + vehicle control. Results are expressed as mean ± SEM (n = 6 mice per group; * p<0.05, ** p<0.01 compared to smoking + NTHi + Scr).