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. 2015 Apr 20;10(4):e0123805. doi: 10.1371/journal.pone.0123805

Fig 2. Effect on protein secretion by MorA in P. aeruginosa is c-di-GMP signaling dependent.

Fig 2

(A) Top panel—Total extracellular protein (ECP) from culture supernatants of P. aeruginosa strains loaded based on protein secreted from equal number of cells. Black arrow indicates the position of elastase (LasB) band. Bottom panel—Immunoblot of RNA polymerase (loading control) on cellular fractions of respective cultures in top panel. L-Ladder (Bio-Rad). (B) Percentage increase in levels of secreted LasB based on protein band quantification by densitometry. Band intensity values of MorA insertion (morA KO) and deletion (ΔmorA) mutants are compared with wildtype (WT) while those of strains expressing MorA with mutations in GGDEF and EAL motifs (represented as ΔmorA–pUG* and ΔmorA–pUE* respectively) are compared with complementation strain (ΔmorA-pU) containing full length morA expressed in ΔmorA background. Error bars represent mean +SE (n = 5). Student’s t-test, p-value<0.05.