Table 1. Bacterial strains and plasmids used in this study.

Strain/ Plasmid	Characteristics		Source/Reference
P. aeruginosa strains
PAO1 WT		Wild-type P. aeruginosa strain	[60]
morA KO		PAO1 with insertional mutation in morA (morAPa::aacC1); Gmr	[56]
ΔmorA		Markerless deletion mutant of morA in PAO1	This study
ΔmorA-pU		ΔmorA complemented with full length morA (pUPMR)	This study
ΔmorA-pUG*		ΔmorA complemented with full length morA containing GGDEF domain mutation (pUE1060K)	This study
ΔmorA-pUE*		ΔmorA complemented with full length morA containing EAL domain mutation (pUE1189K)	This study
fliC KO		PAO1 with insertional mutation in fliC (fliC:: Gmr)	[67]
xcpQ		PAO1 mutant strain PW6222 (xcpQ-C07:: ISphoA/hah)	PA two-allele library [74]
E. coli strains
BL21		F-, ompT, hsdS(r- B, m- B), gal, dcm; host for protein expression	Laboratory collection
DH5α		F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1; host for pK18mobsacB and pK-morAflank	Laboratory collection
Plasmids
pUPMR		Full-length morA Pa gene with native promoter cloned into pUCP19; Ampr	[56]
pRK2013		Vector that aids mobilization of plasmid in triparental conjugation, kanr Gmr	[62]
pK18mobsacB		Allelic exchange suicide plasmid, sucrose-sensitive, kanr Gmr	[61]
pK-morAflank		pK18mobsacB with fusion product containing 5’ upstream and 3’ downstream regions of morA, kanr Gmr	This study
pUE1060K		pUPMR with E1060K mutation; Cbr	This study
pUE1189K		pUPMR with E1189K mutation; Cbr	This study
pETM		Modified pET32 (Novagen) expression vector lacking Trx and S tags	Laboratory collection
pETM-LasB		pETM carrying partial lasB (A198-L498)	This study