Fig 3. Biochemical properties of the xylan XylT activity in microsomes from garden asparagus spears.

Microsomes extracted from the fresh basal Asparagus spears were incubated with UDP-[¹⁴C]-Xyl and Xyl₆ for 1 hr unless otherwise indicated. The XylT activity was measured by counting the radioactivity at the origin of the paper chromatogram and expressed as specific activity. All assays were repeated twice and the values were averaged. Effect of temperature (A), pH (B), reaction time (C), microsomal protein amount (D), amount of Xyl₆ (E) and UDP-Xyl concentration (F) on enzyme activity.