Fig 4. Xylan XylT activity measured using fluorescently tagged (AA) xylo-oligosaccharides of different lengths (Xyl₁-Xyl₆) as exogenous acceptors.

The reaction was conducted by mixing Asparagus microsomes with cold UDP-Xyl and the fluorescent acceptors Xyl₁-AA (B), Xyl₂-AA (C), Xyl₃-AA (D), Xyl₄-AA (F), Xyl₅-AA (G) or Xyl₆-AA (H) and incubated at RT for 1 hr. The reaction products were separated by RP-HPLC and detected by a fluorescence detector. A chromatogram showing a separation of a Xyl₁-AA to Xyl₆-AA mixture of standards is shown in (A) and (E) for reference. The numbers on the plots indicates the DP of the xylo-AA oligosaccharides.