Table 5.
Number of Biopsies from 3 Patients Required Detect a Two-Fold Reduction in Gene Expression
| Inflammatory Gene |
Ileum | Ascending Colon |
Transverse Colon |
Descending Colon |
Rectum |
|---|---|---|---|---|---|
| TNF-α | 2 | 3 | 3 | 3 | 3 |
| IL-1β | 2 | 3 | 3 | 3 | 3 |
| IL-6 | 4 | 6 | 6 | 6 | 6 |
| IL-8 | 4 | 6 | 6 | 6 | 6 |
| S100-A8 | 4 | 5 | 5 | 5 | 6 |
| IP-10 | 4 | 6 | 6 | 6 | 6 |
| MMP-3 | 8 | >9 | >9 | >9 | >9 |
With a linear mixed effect model using repeated measures, the power to detect two-fold reduction in the expression of inflammatory genes from intestinal or colonic biopsies was determined. The minimum number of biopsies prior to and following treatment required from 3 patients to obtain a power of 80% is reported.