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. 2015 Apr 16;7:12. doi: 10.1186/s13099-015-0057-1

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© Amorim et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Helicobacter spp. in canine stomach. A) Numerous spiral-shaped bacteria colonizing the surface epithelium of the gastric pit. HE. Bar = 10 μm; B) Note the intraepithelial lymphocyte infiltration within the deeper gastric glands in the antral mucosa of a NHPH-positive dog. HE. Bar = 20 μm. C) Presence of NHPH inside the parietal cells of the canine gastric body region (black arrow). MG. Bar = 10 μm; D) Large amounts of Helicobacter antigen within the superficial gastric mucus and in the lumen of gastric glands in the body region of canine stomach. Immunoperoxidase-diaminobenzidine stain with Mayer’s haematoxylin counterstain. Bar = 50 μm. Inset shows Helicobacter antigen within parietal cells, sometimes detectable as well preserved spiral-shaped organisms or brown round dots. Immunoperoxidase-diaminobenzidine stain with Mayer’s haematoxylin counterstain. Bar = 10 μm.