Table 2.
Table summarising the histopathological alterations and colonisation density observed in the canine stomach, according to the positive NHPH species-specific PCR results, positive genus results and negative results
| PCR positive results with specific species identification, regardless gastric location (percent&number) (n = 33) | Positive results for Helicobacter spp. (percent&number) (n = 27) | Negative results (percent & number) (n = 9) | p | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Hh | Hf | Hb | Hs | Hf + Hb | Hh + Hs | Hh + Hf | Hb + Hs | Hf + Hb + Hs | ||||
| Histopathology grading | ||||||||||||
| (Day et al., 2008) | ||||||||||||
| Normal | 3.0 (1/33) | 0 | 0 | 3.0 (1/33) | 0 | 0 | 0 | 0 | 0 | 7.4 (2/27) | 11.1 (1/9) | NS |
| Mild gastritis | 15.2 (5/33) | 3.0 (1/33) | 3.0 (1/33) | 6.1 (2/33) | 0 | 18.2 (6/33) | 3.0 (1/33) | 3.0 (1/33) | 6.1 (2/33) | 44.4 (12/27) | 33.3 (3/9) | |
| Moderate gastritis | 12.1 (4/33) | 3.0 (1/33) | 3.0 (1/33) | 0 | 3.0 (1/33) | 9.1 (3/33) | 0 | 0 | 0 | 48.1 (13/27) | 44.4 (4/9) | |
| Marked gastritis | 0 | 0 | 0 | 0 | 0 | 6.1 (2/33) | 0 | 0 | 0 | 0 | 11.1 (1/9) | |
| Epithelial injury | ||||||||||||
| Mild | 27.3 (9/33) | 3.0 (1/33) | 0 | 9.1 (3/33) | 3.0 (1/33) | 30.3 (10/33) | 0 | 3.0 1/3(3) | 6.1 (2/33) | 77.8 (21/27) | 33.3 (3/9) | 0.003 |
| Moderate | 3.0 (1/33) | 3.0 (1/33) | 6.1 (2/33) | 0 | 0 | 0 | 3.0 (1/33) | 0 | 0 | 14.8 (4/27) | 11.1 (1/9) | |
| Fibrosis/mucosal atrophy | ||||||||||||
| Mild | 18.2 (6/33) | 6.1 (2/33) | 6.1 (2/33) | 6.1 (2/33) | 0 | 18.2 (6/33) | 0 | 0 | 3.0 (1/33) | 55.6 (15/27) | 33.3 (3/9) | NS |
| Moderate | 3.0 (1/33) | 0 | 0 | 0 | 3.0 (1/33) | 0 | 0 | 0 | 0 | 3.7 (1/27) | 11.1 (1/9) | |
| Intraepithelial lymphocytes | ||||||||||||
| Mild | 15.2 (5/33) | 3.0 (1/33) | 6.1 (2/33) | 6.1 (2/33) | 0 | 15.2 (5/33) | 0 | 0 | 6.1 (2/33) | 29.6 (8/27) | 11.1 (1/9) | 0.016 |
| Moderate | 3.0 (1/33) | 3.0 (1/33) | 0 | 0 | 3.0 (1/33) | 9.1 (3/33) | 0 | 0 | 0 | 14.8 (4/27) | 0 | |
| Lymphofollicular hyperplasia | ||||||||||||
| Mild | 6.1 (2/33) | 0 | 0 | 0 | 3.0 (1/33) | 0 | 0 | 0 | 0 | 29.6 (8/27) | 11.1 (1/9) | NS |
| Bacterial density (based on the IHC results) | NA | |||||||||||
| + | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14.8 (4/27) | ||
| ++ | 3.0 (1/33) | 0 | 0 | 0 | 3.0 (1/33) | 0 | 0 | 0 | 0 | 25.9 (7/27) | ||
| +++ | 24.2 (8/33) | 6.1 (2/33) | 6.1 (2/33) | 9.1 (3/33) | 0 | 33.3 (11/33) | 3.0 (1/33) | 3.0 (1/33) | 6.1 (2/33) | 51.9 (14/27) | ||
Legend: Hh, H. heilmannii-like; Hf, H.felis; Hb, H. bizzozeronnii; Hs, H. salomonis. Bacterial density: +, few organisms; ++, moderate number of organisms; +++, large number of organisms. NA, not applicable. NS, not significant (p > 0.05).