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. 2015 Apr 20;10(4):e0123562. doi: 10.1371/journal.pone.0123562

Fig 7. Whole-cell recording assay of AMPA receptors expressed in HEK-293S cells grown at 37°C and 33°C.

Fig 7

All of the cells used for the assays were from 48 hours after transfection. (A) Representative traces of 3 mM glutamate induced whole-cell current from HEK-293S cells that transiently transfected with GluA2Q AMPA receptors. The cells cultured at 33°C were maintained at 37°C overnight first. (B) The average current amplitude from GluA2Q channels induced by 3 mM glutamate was shown for each culture. The number of the cells used for the recording was listed on top of each column. The error bar represents standard deviation from the mean. A two sample t-test on the whole-cell current data showed a significant difference between the cells cultured at 33°C and those cultured at 37°C (P value = 0.03). (C) The average whole-cell current amplitude from the GluA3 AMPA receptor channels induced by 3 mM glutamate. The number of cells used for the recording was listed on top of each column. Similarly, a two sample t-test also showed a significant difference between the data collected on the cells cultured at 33°C and those cultured at 37°C (P = 0.02). (D) The effect of BDZ-f on the GluA2Q AMPA receptor whole-cell current amplitude. The cells that expressed the channel were from the 33°C culture. The K I value was found to be 5.1 ± 0.5 μM, determined from the slope (see detail in text).