Fig 3. Functional effects of SERCA1b-specific shRNA transfection on depolarization-evoked calcium transients in C2C12 myotubes.

Representative records of calcium transients evoked by repetitive application of 120 mM KCl in scrambled (A) and in SERCA1b-specific shRNA transfected (B) C2C12 myotubes (cloneC1). Calcium transients were detected in 1.8 mM Ca²⁺-containing Tyrode’s solution. (C-D) Pooled data of amplitudes and rates-of-rise of transients (calculated as maximal d[Ca²⁺]_i/dt) evoked by 120 mM KCl. Numbers in parentheses indicate the number of cells measured. Data represent mean ± standard error of the mean (SEM).