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. 2015 Apr 20;10(4):e0123583. doi: 10.1371/journal.pone.0123583

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© 2015 Tóth et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Representative records showing the difference in maximal transport rate of the Ca²⁺ pump (PV_max) from the declining phase of the Ca²⁺-transient following the KCl-induced depolarization (A) in scrambled and (B) in SERCA1b-specific shRNA transfected C2C12 myotubes (cloneC1). (C) Mean values of [Ca²⁺]_i before and after the transients, demonstrating that following the transients [Ca²⁺]_i declined slower and returned to a significantly higher level in cloneC1 compared to scrambled shRNA transfected cells. (D) Pooled data of SERCA pump activity in selected cloneC1, C5, and scrambled shRNA transfected cells. Numbers in parentheses indicate the number of cells measured. Asterisks (*) and Hashmarks (#) mark significant (P<0.01) differences between cells bathed in standard Tyrode’s solution.