Table 1. List of antibodies using for the detection of different proteins playing important role of calcium homeostasis and differentiation of skeletal muscle.
| name | commercial supplier | catalog number | host | source | dilutions | epitope |
|---|---|---|---|---|---|---|
| MyoD | Santa Cruz | sc-377460 | mouse | monoclonal | 1/200 | amino acids 1–318 of MyoD of mouse origin |
| SERCA1b | Zádor E. Univ. of Szeged | rabbit | monoclonal | 1/800 | terminal octamer of rat SERCA1b | |
| SERCA1 | Thermo Scientific | MA3-912 | mouse | monoclonal | 1/1000 | amino acid 506-C-terminus of rabbit skeletal muscle ATPase |
| SERCA2a | F. Wuytack Univ. of Rotterdam | rabbit | monoclonal | 1/20000 | amino acid 989–997 of pig SERCA2a isoform | |
| Calcineurin | Cell Signaling | 2614S | rabbit | polyclonal | 1/1000 | carboxy terminus of human calcineurin (PP2B) |
| Calsequestrin | Thermo Scientific | PA1-913 | rabbit | polyclonal | 1/1000 | purified canine cardiac calsequestrin |
| STIM1 | BD Laboratories | 610954 | mouse | monoclonal | 1/500 | amino acid 25–139 human |
| Actin | Santa Cruz | sc-1616 | rabbit | polyclonal | 1/500 | C-terminus of Actin of human origin |
| Desmin | Sigma Aldrich | D1033 | mouse | monoclonal | 1/500 | pig stomach was used as the immunogen |
| Anti-Ca v pan | alomone | ACC-004 | rabbit | polyclonal | 1/200 | C-terminus of rat Cav1.2 |