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. 2015 Apr 20;11(4):e1004161. doi: 10.1371/journal.pcbi.1004161

Fig 6. RUNX1 is required for mammary stem cells to differentiate into tissue rudiments in 3D culture.

Fig 6

(A) Brightfield images of day 8 shRUNX1 (sh1, sh2, sh3) or control (shLuc) MCF10A cells seeded into collagen culture. % KD denotes the percent of expression lost relative to shLuc control as quantified by qPCR. (B) Western blots quantifying the percentage of expression lost relative to shLuc control at the protein level. (C) Quantification of tissue rudiments formed by shRUNX1 and shLuc cells. CellProfiler was utilized to quantify total ducts and lobules as well as ductal features (long-axis length, structure area, structure perimeter). Error bars denote SEM from 10 fields per condition. (D) Confocal microscopy of tissue rudiments of shLuc and shRUNX1 cells, taken 8 days after seeding into collagen. Dashed line indicates the hollowed region of a mature lobule.