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. 2015 Apr 20;10(4):e0124992. doi: 10.1371/journal.pone.0124992

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 2 — (A) The virus growth was calculated as the fold increase at different time points compared with the 12^th h post infection. The asterisk indicates significant differences of the growth kinetics between rHVT-pmpD-N and parental HVT (P<0.05). (B) The replication kinetics of parental HVT (n = 10) and rHVT-pmpD-N (n = 15) in vaccinated chickens was evaluated by real time PCR in PBLs. The HVT genome copies were quantified in 75 ng extracted DNA at different time point. The values were shown as means ± standard deviation.