Table 1. The primers to amplify the coding exons and flanking introns of PITX2.

Exon	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Amplicon (bp)
2	GAGGCTAGGCTGGAGATGCT	CCACTGGCGATTTGGTTCTG	385
3	TTGCTCTTTGTCCCTCTTTC	CCAGAGGCGGAGTGTCTAAG	399
4	CAGCTTGGCTTGAGAACTCG	TGACTTCCTTGGGGCGAGAG	442
5	CAGCTCTTCCACGGCTTCTG	GCTGCCTTCCACATTCTCTC	387
6	AATCTGCACTGTGGCATCTG	AGTCTTTCAAGGGCGGAGTT	677