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. 2015 Apr 20;10(4):e0124270. doi: 10.1371/journal.pone.0124270

Fig 2. The tgrC1 - sporulation defect is non-cell autonomous and the stalk development defect is cell-autonomous.

Fig 2

A. We mixed tgrC1− with tgrC1−stcAins or AX4, as indicated, at 1:1 ratios, and developed the mixture for 72 hours. We collected the spores, germinated them in association with Klebsiella aerogenes to allow single plaque formation and recorded the fraction (%) of tgrC1− plaques relative to the total number of plaques. The average fraction and the s.d. are shown (y-axis; n = 3). The overall sporulation efficiency of the tgrC1− and tgrC1−stcAins strains was similar to that of tgrC1−stcAins alone (Fig 1B). The overall sporulation efficiency of the mix between tgrC1− and AX4 was about half of that of AX4 alone (Fig 1B). We mixed GFP-labeled tgrC1− (green text) with RFP-labeled AX4 or tgrC1−stcAins (red text) and developed the mixture of cells on non-nutrient agar. We photographed the structures at 16 hour (B) and single fruiting bodies of GFP-labeled tgrC1− and RFP-labeled tgrC1−stcAins at 48 hour (C). Scale bar, 0.2 mm.