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. 2015 Mar 10;12(2):477–490. doi: 10.1007/s13311-015-0343-0

Fig. 5.

Fig. 5

The effect of pentobarbital (PB) on reversal of cellular and functional defects caused by lost of methyl-CpG-binding protein 2 (MeCP2). Cells were grown for 24 h before treatment with vehicle or 5 μM PB. Culture media were replaced with fresh media with drugs every other day throughout the entire culture period (12–14 days in vitro). (a–c) Total dendritic length, branch number, and density of synapsin-1-positive puncta per μm dendrite in hippocampal neurons transfected with scrambled RNA (shctrl) or short hairpin RNA against MeCP2 (shMeCP2), treated with vehicle (V) or PB. n.s = not significant (**p < 0.01; analysis of variance–Newman–Keuls multiple comparison test). (d–f) (d) Representative traces and graphs showing quantitative analysis of (e, f) the frequency and the amplitude of miniature excitatory postsynaptic currents (mEPSCs) in hippocampal neurons transfected with shctrl or shMeCP2, treated with vehicle or PB n.s = not significant (***p < 0.01; analysis of variance–Newman–Keuls multiple comparison test). (g, h) Quantitative analysis of (g) the frequency and (h) the amplitude of mEPSCs in hippocampal neurons expressing shctrl or shMeCP2, treated with vehicle (V), 5 μM bicuculline (BMI), or 25 μM muscimol (Mus) (**p < 0.01; analysis of variance–Newman–Keuls multiple comparison test)