Fig. 7.

Pentobarbital (PB) rescues methyl-CpG-binding protein 2 (MeCP2)-mediated inhibition of dendritic development in newborn dentate gyrus granule cells in adult mice. Mice received intraperitoneal injections of pentobarbital (1 mg in 100 μl saline per mouse) every 2 days after virus injection, until day 14, when they were euthanized for analysis. (a) Schematic diagram showing detailed time line of the experimental procedure for chronic PB treatment. (b, c) Quantitative analysis of the total dendritic length, and (d, e) total branch number of control or MeCP2-deficient neurons treated with vehicle (V) or PB. n.s = not significant (***p < 0.01; analysis of variance–Newman–Keuls multiple comparison test)