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. Author manuscript; available in PMC: 2015 Apr 21.
Published in final edited form as: Clin Chem. 2014 Nov 20;61(1):213–220. doi: 10.1373/clinchem.2014.231456

Table 1.

Overall performance of exome enrichment methods.

Capture method Base pairs targeted by method (megabases) Sequencer Unique samples examined Mean uniquely mapped reads (millions) Mean total base pairs generated (gigabases) Mean base pairs generated in target regions (gigabases) Mean read depth in target regions Mean percentage of targeted coding nucleotides by depth of coverage (%)a
>5× >10× >20×
SureSelect v4 (Agilent) 70 SOLiD 5500xl 12 199 7.6 3.4 120× 95 92 87
TargetSeq (LifeTech) 37 SOLiD 5500xl 33 206 6.7 2.8 76× 91 89 83
TruSeq (Illumina) 62 HiSeq 2000 12 92 9.1 4.5 74× 93 91 84
a

SureSelect v4 is designed to target 70 MB including noncoding regions such as untranslated regions; the analysis for coding nucleotide coverage was limited to the 50 MB of coding nucleotides targeted by the method.