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. 2015 Apr 16;14:57. doi: 10.1186/s12934-015-0241-5

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© Mamat et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Charge deconvoluted ESI FT-ICR mass spectra in negative ion mode of lipid IV_A isolated from BW30270-derived mutants. The lipid IV_A (calculated mass 1404.854 u) was extracted from E. coli (K-12) strains KPM318 (A) and KPM335 (B). Mass numbers given refer to the monoisotopic masses of neutral molecules. Peaks representing molecules with variations in acyl chain length are labeled with asterisks (∆m = 14.02 u). The molecular ion at 1484.82 u in panel B indicates the presence of a minor fraction of 1-diphosphate lipid IV_A. The structures of lipid IV_A and 1-diphosphate lipid IV_A are shown as insets in panels A and B, respectively.