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. 2015 Mar 19;16(1):54. doi: 10.1186/s13059-015-0627-z

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© Hughes et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overview of total gene expression variation at 13156 expressed genes. (A) A cluster dendrogram of libraries based on the following expression distance between each pair of libraries: 1-abs(r), where r is Pearson’s correlation coefficient for expression levels across all genes. Individual libraries and branches are colored to designate their group affiliation; asterisks indicate three pairs of replicate libraries that do not cluster together. (B) Scatter plots of the first three principle components (PC) using data from individuals and all genes. The explained proportion of variation is annotated on each axis. (C) Scatter plots between the first few PCs and correlated explanatory variables.