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. 2015 Mar 21;6(1):41. doi: 10.1186/s13287-015-0042-0

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© Li et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Differentiation of NSC-OPCs. The cells that migrated out of the oligospheres were labeled with antibodies and DAPI. (A) Cell staining with anti-PDGFRα antibody (red); (B) Cell staining with anti-nestin antibody (green); (C) Nuclei staining with DAPI, (D) Merged image of images (A)–(C), (E) Cell staining with anti-nestin antibody (green), (F) Cell staining with anti-A2B5 antibody (green), (G) Nuclei staining with DAPI, (H) Merged image of images (E)–(G). (E’–H’) Magnified images of inset indicated in (E–H), respectively. Scale bar: 100μm.