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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Biochemistry. 2015 Feb 26;54(9):1849–1857. doi: 10.1021/bi501491z

Figure 6.

Figure 6

ICL breakdown due to BER enzymes. A. Duplex 2/3 ICL disappears over time in reactions containing 0.5 µM APE1 or 0.5 µM Nth; exponential fits to the data give values of kobs of 0.05 min−1 and 0.03 min−1 for the reactions containing APE1 and Nth, respectively. B. Duplex 2/3 ICL disappears with kobs= 0.03 min−1 in reactions containing either 0.5 µM AAG or 0.5 µM AlkA. For both A and B, BER enzymes were added to 0.25 µM duplex 2/3 at equilibrium at 37 °C and pH 6.5, and samples were reduced with NaBH4 prior to separation on 20% denaturing polyacrylamide gels (Figure S2). APE1 and Nth reactions included 0.25 mM MgCl2.