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. 2015 Apr 9;11:26. doi: 10.1186/s13007-015-0070-x

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© Heckwolf et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Applying the tool to transections of Miscanthus gigantum stalks. A) Stalk perimeter (blue line) and rind/pith boundary (red line) superimposed on the orginial, unprocessed color image. B) Detected vascular bundles (red circles). C) An individual vascular bundle in the unprocessed image. D) Grayscale representation of A after processing including homomorphic filtering. E) Level contours of the 2D Gaussian distribution fit to the grayscale image in D projected onto the original image. This shows the method measures the very small vascular bundles in thin stalks of Miscanthus.