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. 2015 Apr 21;5:9956. doi: 10.1038/srep09956

Figure 8. Kinetics of FC85 and/or ISA27 inhibition of AKT and ERK1/2 phosphorylation.

Figure 8

U87MG-derived CSCs were treated for the indicated times (0–120 min) with NSC medium containing DMSO (control), or 500 nM FC85, or 2.5 µM ISA27, alone or in combination. At the end of the treatment periods, the levels of AKT (a) and ERK 1/2 (b) phosphorylation were evaluated using ELISA kits, as described in the Methods section. The data are expressed as the percentage of phosphorylated AKT or ERK1/2 relative to those of untreated cells (control), which were set at 100%, and are the mean values ± SEM of three independent experiments performed in triplicate. The significance of differences was performed using one-way ANOVA with Bonferroni post-test: * p< 0.05, ** p< 0.01, *** p<0.001 vs. control; # p<0.05, ##p< 0.01 vs. FC85 alone; § p<0.05, §§ p<0.01, §§§ p<0.001 vs. ISA27 alone.