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. 2015 Apr 21;6:246. doi: 10.3389/fpls.2015.00246

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Copyright © 2015 Vogler, Konrad and Sprunck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Arabidopsis pollen imaging in the micro-germination setup. Mounting pollen in a single (A), or multi well (B), micro-germination setup for live cell imaging. In the center of one well (C) PTs grow in close proximity to the cover slip (D), allowing the use of high NA immersion objectives with low working-distances. Pollen germination is not affected by the mounting technique and PTs showed normal morphology (E). A time series of germinating P_Lat52:GFP pollen is shown in (F). Brightfield images are shown as sum slice projections and confocal fluorescence images of cytoplasmic GFP as maximum intensity projections. Binary images of GFP channel enable unbiased quantitative measurement of PT area size and morphology (F). Scale bars: (C,D) 200 μm, (E) 20 μm, (F) 50 μm.