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. 2015 Apr 21;6:258. doi: 10.3389/fpls.2015.00258

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Copyright © 2015 Pontiggia, Ciarcianelli, Salvi, Cervone, De Lorenzo and Mattei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recovery of OGs. (A) Recovery from 5-week-old leaves (50 mg) infiltrated with fluorescent labeled OGs (OG-6A, 25 μg at 100 ng/μl) or added with OG-6A (25 μg) before and after precipitation with 80% ethanol. (B) Recovery from leaf tissue (50 mg) infiltrated with 250 μl of unlabeled OGs at different concentrations (100, 250, and 500 ng/μl, corresponding to 25, 62.5, and 125 μg, respectively, of total OGs) after 80% ethanol precipitation. Each column represents the mean ± SE of three independent experiments.