Figure 1.

Daily HBO therapy suppresses RANKL-induced osteoclast formation in a treatment number-dependent manner. Human PBMCs were incubated in the presence of RANKL (30 ng/mL) and M-CSF (50 ng/mL), stained for TRAP, and the total number of TRAP-positive osteoclasts quantified. Representative images of RANKL-treated TRAP-stained cultures from pre-HBO group (panel A), 10 HBO exposures (B), and 25 HBO exposures (C) (magnification ×40). Arrows show examples of multinucleated osteoclasts and arrowheads show examples of mononuclear osteoclasts. Data are expressed as mean ± SEM (n = 6).