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. 2015 Apr 21;6:197. doi: 10.3389/fpls.2015.00197

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Copyright © 2015 McDowell, López-Marqués, Cohen, Brown, Rosenberg, Palmgren and Harper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Loss of ALA6 and ALA7 reduces the ability of lipophilic FM dyes to stain living pollen tubes. (A) Confocal micrographs showing DIC and FM dye emission for wild-type and ala6-1/7-2 pollen tubes stained with FM4-64. (B) Quantitative analysis of FM dye emission from pollen tubes stained with FM4-64 and FM1-43. Average results (±SE) are reported for three independent experiments, n = 21–36 pollen tubes. Pollen tubes were collected from a total of 5–11 different plants for each genotype and dye. For the GFP-ALA6 rescue construct, 4/4 lines showed equivalent rescue of ala6-1/7-2: ss1878, ss1880, ss1881, ss1883. For the ALA6-YFP rescue construct, 4/4 lines showed equivalent rescue of ala6-1/7-2: ss1885–1888. ^*Statistically significant difference between wild-type and ala6-1/7-2 (Welch's t-test, p < 0.05).