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. 2015 Feb 25;4(2):e978709. doi: 10.4161/2162402X.2014.978709

Figure 2.

Figure 2.

Temozolomide treatment induces T-cell infiltration into genitourinary tumors in a model of spontaneous melanoma. (A-C) RETAAD mice were treated with Temazolomide (TMZ) only or dimethyl sulfoxide (DMSO) vehicle after they were determined to have developed genitourinary (GU) and cutaneous tumors by clinical examination. (A) qRT-PCR analysis was performed to examine for gene expression of CD3, CD4 and CD8 in GU tumors from RETAAD mice following TMZ treatment. (B) Flow cytometry analysis of T cells in GU tumors from RETAAD mice at day 10 after TMZ treatment. (C) Immunofluorescence imaging to detect T cells in GU tumors from RETAAD mice at day 10 after TMZ treatment. Data from (A) are pooled from 3-5 mice per group and data from (B) are pooled from 2 separate experiments with 3-4 mice per group in each experiment (n = 6-8 in each group). Bars represent mean ± SD. Statistical analyses were performed using one-way ANOVA test with Bonferroni's post-test analysis in (A) and the unpaired two-tailed Student's t-test in (B); *p<0.05, **p<0.01. Images from (C) are representative of 5 independent experiments (n = 5 in each group). Scale bars in (C) represent 200 μm.