Figure 9.

TMZ-induced T cell infiltration inhibits tumor growth. C57/BL6 wild type (WT) and Cxcr3^−/− mice were injected subcutaneously in each flank with 10⁶ Melan-ret cells and treated with either 2 mg Temozolomide (TMZ) or vehicle [dimethyl sulfoxide (DMSO)] daily for 3 days once tumors became palpable. (A) Growth kinetics of transplanted Melan-ret tumors in WT or Cxcr3^−/− mice treated with DMSO or TMZ. (B) Frequency of tumor cell death measured by the proportion of Annexin⁺ AQUA⁺ CD45⁻ tumor cells and quantified by flow cytometry analysis. Data from panel (A) are pooled from 8-10 mice per group and data from (B) are pooled from 5 mice per group. Bars represent mean ± SD. Statistical analyses were performed using one-way ANOVA test with Bonferroni's post-test analysis; *p<0.05, **: p<0.01.