Table 5.
Systemic immune changes according to sentinel lymph node invasion (upper part) and sentinel IDO-positivity (lower part)
| Sentinel invaded | Sentinel non-invaded | ||||||
|---|---|---|---|---|---|---|---|
| Median | IQR | Min–max | Median | IQR | Min–max | p-value* | |
| CTLA-4 expression by Tregs (%) | 93.2 | 90.8–96.3 | 88.7–99.0 | 91.6 | 88.8–93.9 | 83.2–99.7 | 0.045 |
| MFI for CTLA-4 in Tregs | 1931 | 1720–2126 | 1261–2630 | 1695 | 1449–1955 | 1179–2985 | 0.036 |
| pDCs (% of live PBMCs) | 0.38 | 0.29–0.52 | 0.16–1.01 | 0.28 | 0.22–0.44 | 0.13–0.75 | 0.034 |
| Sentinel IDO-positive | Sentinel IDO-negative | ||||||
| Median | IQR | Min–max | Median | IQR | Min–max | p-value** | |
| IDO+ PBMCs (%) | 0.64 | 0.24–1.22 | 0.11–2.57 | 0.39 | 0.14–0.53 | 0.015–1.86 | 0.016 |
| IDO+ mMDSCs (%) | 0.60 | 0.16–1.29 | 0.00–14.10 | 0.095 | 0.035–0.60 | 0.00–1.52 | 0.018 |
CTLA-4, cytotoxic T-lymphocyte-associated antigen 4; IDO, indoleamine 2,3-dioxygenase; IQR, interquartile range; PBMC, peripheral blood mononuclear cell; pDC, plasmacytoid dendritic cell; Treg, regulatory T-cell. * p-values indicate significance of the difference between patients with invaded and non-invaded sentinel lymph nodes (Mann–Whitney-U Test). ** p-values indicate the significance of the difference between patients with IDO-positive and IDO-negative lymph nodes (Mann–Whitney-U Test).