Table 2.

RBC membrane protein composition, band 3 profile of and membrane-bound hemoglobin throughout the storage of RBC units under standard blood bank conditions

	D0	D7	D14	D21	D28	D35	D42
Band 3 profile
HMWAg, %	12.26 ± 7.16	12.36 ± 7.84	10.98 ± 7.22	11.70 ± 7.11	11.09 ± 8.18	12.19 ± 8.14	11.88 ± 7.97
Band 3 monomer, %	75.43 ± 7.43	75.14 ± 8.88	75.93 ± 8.86	75.22 ± 6.85	75.02 ± 7.99	74.08 ± 8.48	76.10 ± 10.43
Pfrag, %	12.98 ± 9.45	13.11 ± 7.57	13.73 ± 5.95	13.98 ± 6.51	14.54 ± 7.63	14.46 ± 6.65	12.48 ± 8.82
HMWAg/Bd3	0.16 ± 0.10	0.17 ± 0.12	0.15 ± 0.11	0.16 ± 0.11	0.16 ± 0.13	0.17 ± 0.12	0.17 ± 0.12
Pfrag/Bd3	0.17 ± 0.16	0.17 ± 0.13	0.18 ± 0.12	0.18 ± 0.11	0.19 ± 0.13	0.19 ± 0.11	0.17 ± 0.15
RBC membrane protein composition
Spectrin, %	29.96 ± 4.60	28.36 ± 5.89	26.72 ± 4.96a	28.93 ± 4.26	28.62 ± 6.37	29.26 ± 6.29	28.79 ± 5.83
Ankyrin, %	6.44 ± 1.70	6.20 ± 1.57	5.91 ± 1.21	5.64 ± 1.25	5.66 ± 1.20	5.49 ± 1.96ab	5.59 ± 1.81b
Band 3, %	29.29 ± 6.55	28.72 ± 6.87	30.87 ± 7.30b	29.05 ± 6.82	30.20 ± 7.57	31.83 ± 5.92b	30.69 ± 6.58
Protein 4.1, %	7.97 ± 1.59	7.84 ± 1.08	7.36 ± 2.17	7.73 ± 1.38	8.17 ± 1.39	7.30 ± 1.07	7.82 ± 1.06
Protein 4.2, %	6.01 ± 1.22	6.27 ± 1.59	6.86 ± 1.34a	6.94 ± 2.53	6.27 ± 1.10	6.02 ± 1.05	6.37 ± 0.99
Band 5, %	9.18 ± 2.25	9.77 ± 1.22	9.46 ± 1.45	9.68 ± 1.93	8.93 ± ± 1.46	9.11 ± 0.99	9.10 ± 1.21b
Band 6, %	9.14 ± 2.20	10.33 ± 2.61	10.06 ± 2.66	9.45 ± 1.91	9.65 ± 2.26	8.32 ± 1.59b	9.29 ± 1.86
Band 7, %	2.02 ± 0.55	2.51 ± 0.91a	2.77 ± 0.92ab	2.59 ± 0.86a	2.50 ± 0.74a	2.66 ± 1.17a	2.34 ± 1.14
Protein 4.1/spectrin	0.27 ± 0.07	0.29 ± 0.08	0.28 ± 0.10	0.27 ± 0.06	0.30 ± 0.09	0.26 ± 0.09	0.28 ± 0.08
Protein 4.1/band 3	0.29 ± 0.10	0.29 ± 0.08	0.26 ± 0.13	0.28 ± 0.10	0.29 ± 0.08	0.24 ± 0.06be	0.27 ± 0.10
Protein 4.2/band 3	0.21 ± 0.04	0.22 ± 0.04	0.23 ± 0.08	0.26 ± 0.14	0.21 ± 0.04	0.19 ± 0.03ce	0.22 ± 0.05
Spectrin/band 3	1.09 ± 0.36	1.10 ± 0.54	0.95 ± 0.48	1.06 ± 0.37	1.06 ± 0.54	0.98 ± 0.39	1.02 ± 0.45
Ankyrin/band 3	0.24 ± 0.10	0.24 ± 0.13	0.21 ± 0.10	0.21 ± 0.09	0.21 ± 0.09	0.19 ± 0.10ab	0.20 ± 0.12b
Spectrin/ankyrin	4.84 ± 0.99	4.65 ± 0.50	4.59 ± 0.73	5.27 ± 0.94bc	5.19 ± 1.18	5.77 ± 1.74abc	5.45 ± 1.42
Membrane bound hemoglobin
MBH × 10–4, %	39.63 ± 26.92	31.00 ± 14.29	38.78 ± 18.00	35.11 ± 26.66	43.56 ± 28.09	41.67 ± 23.53	38.88 ± 24.21

HMWAg = High molecular weight aggregates; Pfrag = proteolytic fragments MBH = membrane-bound hemoglobin. *Data presented as mean and standard deviation.

^ap < 0.05, vs. D0.

^bp < 0.05, vs. D7.

^cp < 0.05, vs. D14.

^dp < 0.05, vs. D21.

^ep < 0.05, vs. D28.

^fp < 0.05, vs. D35.